Students take introductory courses in genetics, evolutionary biology, math, inorganic & organic chemistry, and biochemistry, and may choose from a variety of upper level courses in cellular biology, microbiology, plant breeding, and others. Laboratory courses focus on molecular genetics, evolutionary genetics, and genomics.
The oxidation of a ketone by a peroxy acid to yield an ester. This reaction is catalyzed by bacterial
monooxygenases and has proved useful in preparing optically pure esters and lactones. See Ryerson,
C.C., Ballou, D.P., and Walsh, C., Mechanistic studies on cyclohexanone oxygenase, Biochemistry
21, 2644–2655, 1982; Bolm, C., Metal-catalyzed asymmetric oxidations, Med. Res. Rev. 19,
348–356, 1999; Zambianchi, F., Pasta, P., Carrea, G. et al., Use of isolated cyclohexanone monooxygenase from recombinant Escherichia coli as a biocatalyst for Baeyer–Villiger and sulfide oxidations, Biotechnol. Bioeng. 78, 489–496, 2002; Alphand, V., Carrea, G., Wohlgemuth, R. et al.,
Towards large-scale synthetic application of Baeyer–Villiger monooxygenase, Trends Biotechnol.
21, 318–323, 2003; Walton, A.Z. and Stewart, J.D., Understanding and improving NADPH-dependent reactions by nongrowing Escherichia coli cells, Biotechnol. Prog. 20, 403–411, 2004; Malito,
E., Alfieri, A., Fraaije, M.W., and Mattevi, A., Crystal structure of a Baeyer–Villiger monooxygenase, Proc. Natl. Acad. Sci. USA 101, 13157–13162, 2004; ten Brink, G.J., Arends, I.W., and
Sheldon, R.A., The Baeyer–Villiger reaction: new developments toward greener procedures, Chem.
Rev. 104, 4105–4124, 2004; Boronat, M., Corma. A., Renz, M. et al., A multisite molecular
mechanism for Baeyer–Villiger oxidations on solid catalysts using environmentally friendly H2O2
as oxidant, Chemistry 11, 6905–6915, 2005; Mihovilovic, M.D., Rudroff, E., Winninger, A. et al.,
Microbial Baeyer–Villiger oxidation: stereopreference and substrate acceptance of cyclohexanone
monooxygenase mutants prepared by directed evolution, Org. Lett. 8, 1221–1224, 2006; Baldwin,
C.V. and Woodley, J.M., On oxygen limitation in a whole cell biocatalytic Baeyer–Villiger oxidation
process, Biotechnol. Bioeng. 95, 362–369, 2006.
This reagent was developed to improve the reactivity of PMSF. It was originally considered to be somewhat more
effective than PMSF; however, AEBSF has been shown to be somewhat promiscuous in its reaction pattern and care
is suggested in its use during sample preparation. See Su, B., Bochan, M.R., Hanna, W.L. et al., Human granzyme
B is essential for DNA fragmentation of susceptible target cells, Eur. J. Immunol. 24, 2073–2080, 1994; Helser, A.,
Ulrichs, K., and Muller-Ruchholtz, W., Isolation of porcine pancreatic islets: low trypsin activity during the isolation
procedure guarantees reproducible high islet yields, J. Clin. Lab. Anal. 8, 407–411, 1994; Dentan, C., Tselepis, A.D.,
Chapman, M.J., and Ninio, E., Pefabloc, 4-[2-aminoethyl’benzenesulfonyl fluoride, is a new potent nontoxic and
irreversible inhibitor of PAF-degrading acetylhydrolase, Biochim. Biophys. Acta 1299, 353–357, 1996; Sweeney, B.,
Proudfoot, K., Parton, A.H. et al., Purification of the T-cell receptor zeta-chain: covalent modification by 4-(2aminoethyl)-benzenesulfonyl fluoride, Anal. Biochem. 245, 107–109, 1997; Diatchuk, V., Lotan, O., Koshkin, V.
et al., Inhibition of NADPH oxidase activation by 4-(2-aminoethyl)benzenesulfonyl fluoride and related
compounds, J. Biol. Chem. 272, 13292–13301, 1997; Chu, T.M. and Kawinski, E., Plasmin, subtilisin-like
endoproteases, tissue plasminogen activator, and urokinase plasminogen activator are involved in activation of latent
TGF-beta 1 in human seminal plasma, Biochem. Biophys. Res. Commun. 253, 128–134, 1998; Guo, Z.J., Lamb, C.,
and Dixon, R.A., A serine protease from suspension-cultured soybean cells, Phytochemistry 47, 547–553, 1998;
Wechuck, J.B., Goins, W.F., Glorioso, J.C., and Ataai, M.M., Effect of protease inhibitors on yield of HSV-1-based
viral vectors, Biotechnol. Prog. 16, 493–496, 2000; Baszk, S., Stewart, N.A., Chrétien, M., and Basak, A., Aminoethyl
benzenesulfonyl fluoride and its hexapeptide (AC-VFRSLK) conjugate are both in vitro inhibitors of subtilisin kexin
isozyme-1, FEBS Lett. 573, 186–194, 2004; King, M.A., Halicka, H.D., and Dzrzynkiewicz, Z., Pro- and anti-apoptotic
effects of an inhibitor of chymotrypsin-like serine proteases, Cell Cycle 3, 1566–1571, 2004; Odintsova, E.S., Buneva,
V.N, and Nevinsky, G.A., Casein-hydrolyzing activity of sIGA antibodies from human milk, J. Mol. Recog. 18,
413–421, 2005; Solovyan, V.T. and Keski-Oja, J., Proteolytic activation of latent TGF-beta precedes caspase-3
activation and enhances apoptotic death of lung epithelial cells, J. Cell Physiol. 207, 445–453, 2006.
This book evolved out of the process of revising The Practical Handbook of Biochemistry and Molecular Biology, which was edited by the late Gerald Fasman. I had come to several conclusions: (1) I no longer understood the titles of articles in journals because such titles were increasingly written in tongues only understood by selected tribes of investigators; (2) I had forgotten most of the organic chemistry passed on to me by distinguished individuals including Chuck Anderson, Stan Moore, and Bill Stein; (3) many investigators now worked with kits of stuff and had little knowledge of the stuff; and (4) I was not alone with respect to (1) and (2). The enclosed material has been assembled to supplement The Practical Handbook of Biochemistry and Molecular Biology. The content is biased toward my own particular interests and I would appreciate receiving comment regarding this speciﬁc issue. While I spent considerable time reading journals such as The Journal of Biological Chemistry, Biochemistry, The Journal of Molecular Biology, and Nucleic Acids Research from cover to cover and selecting terms and acronyms that I did not readily recognize for inclusion, I do recognize that the selected content will seem weak or incomplete in certain areas. In particular, I would appreciate guidance on acronyms: The list of accepted abbreviations appears to be an item of the past and authors are allowed to indulge their individual creativity in creating new and novel acronyms that, in turn, lead to interesting search results when using Internet search engines. The same holds for the invention of new terms to describe old phenomena; in general, biomedical investigators are not very good at brand naming. Finally, I urge you to visit your local library. The amount of material that you can get from sitting in front of your computer is limited with respect to what you get with focused searches guided by an experienced reference librarian. It is not unlike the situation in the late Douglas Adams’s The Hitchhiker’s Guide to the Galaxy, where an answer is meaningless unless you thoroughly understand the question.
Essays in biochemistry volume 24 Edited by R …
What are the general goals of bioethics? As a discipline of applied ethics and a particular way of ethical reasoning that substantially depends on the findings of the life sciences, the goals of bioethics are manifold and involve, at least, the following aspects:
(2003) Essays in Biochemistry Volume 39-43
How do evolutionists respond to the zero likelihood of life arising by chance? The biochemistry text quoted above asks and then answers the question: “How then did life arise? The answer, most probably, is that it was guided according to the Darwinian principle of the survival of the fittest as it applies at the molecular level.” The key fact to note here is that natural selection simply cannot act unless there are functional, self-replicating molecules present to act on. We have already seen that no such system could possibly appear by chance. Life in its totality must have been created in the beginning, just as God told us.
Early Adventures in Biochemistry - Volume 1 of Foundations ..
Can any scientist with a Ph.D. believe in the idea of a literal six-day creation? answers this provocative question with 50 informative essays by scientists who say “Yes!” Taking a factual and scientific look at the evidence for evolution, physicists, biologists, and chemists conclude that evolution may offer no more evidence than traditional religion, and factually, it may lag behind.